Consumables and method to separate and quantitate

Caffeine

Column
new
Column
Newcrom B
Phase (solvent)
MeCN/H2O - 40/60%
Buffer
MeOH/H2O/HClO4 - 50/50/0.1%
100ml
Standard
EDTA
45ml
Flow rate
0.70 mL/min
Run time
5 min
Standard chromatogram

How to use applications

See full software guide
  1. Order all consumables specified in the application: column, mobile phase, standard.
  2. Add method preset to your hplc.cloud cabinet.
  3. After you receive your consumables, use the method to create a sequence.
  4. Install the mobile phase and column on your Alltesta.
  5. Run a calibration with the standard sample.
  6. Run your sample of interest.