Consumables and method to separate and quantitate

Vitamin B2, 8-Hydroxyquinoline

Column
Primesep B2
4.6x50 mm,
Column
Primesep AP
4.6x50 mm, 5 µm, 100 A
Buffer
MeOH/H2O/HClO4 - 50/50/0.1%
100ml
Phase (mix)
MeCN/H2O/H2SO4 - 60/40/0.4%
Standard
Nicotinic Acid
70ml
Flow rate
0.40 mL/min
Run time
10 min
Standard chromatogram

How to use applications

See full software guide
  1. Order all consumables specified in the application: column, mobile phase, standard.
  2. Add method preset to your hplc.cloud cabinet.
  3. After you receive your consumables, use the method to create a sequence.
  4. Install the mobile phase and column on your Alltesta.
  5. Run a calibration with the standard sample.
  6. Run your sample of interest.