4-Amino-2-nitrophenol is a hydrocarbon with the molecular formula C6H6N2O3. It is a red-purple powder that is typically used in as an oxidation base in hair dye. California considers this compound a carcinogen. 4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
The HPLC Method for Analysis of 4-Amino-2-nitrophenol on Alltesta™ Gradient is designed for 4-Amino-2-nitrophenol, a hydrocarbon compound. 4-Amino-2-nitrophenol is a hydrocarbon with the molecular formula C6H6N2O3.
Run the separation on Primesep 100 (3.2 x 150 mm, 5 um, 100 A, dual ended), SKU 100-32.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 0.5. Injection volume: 2. Run time: 10.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
