HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient
6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation. 6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
Frequently Asked Questions: HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient
What analyte does the HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient cover?
The HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient is designed for 6-Benzylaminopurine, a synthetic cytokinin compound. 6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5.
Which column and mobile phase are required for 6-Benzylaminopurine analysis?
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
What instrument conditions should I use for the HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient?
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 10.