HPLC MethodAlltesta™ GradientSynthetic Cytokinin275 nm

HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation. 6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

Chromatogram

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Chromatogram for 6-Benzylaminopurine
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Flow Rate
1ml/min
Injection Volume
1µL
Run Time
10min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
H
H2SO4
0.2%

Frequently Asked Questions: HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient cover?

The HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient is designed for 6-Benzylaminopurine, a synthetic cytokinin compound. 6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5.

Which column and mobile phase are required for 6-Benzylaminopurine analysis?

Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of 6-Benzylaminopurine on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 10.

Sample Information

Structure of 6-Benzylaminopurine
Analyte
6-Benzylaminopurine
Category
Synthetic Cytokinin

Column Information

Primesep 100 column

Primesep 100

100-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025