High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen (Paracetamol) Acetaminophen, also known as Paracetamol, is a medication with the molecular formula C8H9NO2. It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug. Acetaminophen (Paracetamol) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
The HPLC Method for Analysis of Acetaminophen (Paracetamol) on Alltesta™ Gradient is designed for Acetaminophen (Paracetamol), a organic compound compound. High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen (Paracetamol) Acetaminophen, also known as Paracetamol, is a medication with the molecular formula C8H9NO2.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 10% 10% H2SO4, 0.2%; H2O 90%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 8.
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Last updated: Dec 3, 2025
