Adenine is a chemical compound with the molecular formula C5H5N5. It is a building block for DNA and RNA as one of the four essential nucleotide bases. It is vital as a part of the main energy-carrying molecule in cells. When in DNA, it pairs with thymine, while in RNA, it pairs with uracil. Adenine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
The HPLC Method for Analysis of Adenine on Alltesta™ Gradient is designed for Adenine, a nucleotide base compound. Adenine is a chemical compound with the molecular formula C5H5N5.
Run the separation on Primesep 100 (3.2 x 150 mm, 5 um, 100 A, dual ended), SKU 100-32.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.1%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 0.5. Injection volume: 1. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
