HPLC MethodAlltesta™ GradientAntibiotic275 nm

HPLC Method for Analysis of Amoxicillin on Alltesta™ Gradient

Amoxicillin is a prescription antibiotic with the molecular formula C16H19N3O5S. It is typically used to treat bacterial infections and is taken orally. Amoxicillin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

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Chromatogram

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Chromatogram for Amoxicillin
Flow Rate
0.5ml/min
Injection Volume
2µL
Run Time
17min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
H
H2SO4
0.1%
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Frequently Asked Questions: HPLC Method for Analysis of Amoxicillin on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Amoxicillin on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Amoxicillin on Alltesta™ Gradient is designed for Amoxicillin, a antibiotic compound. Amoxicillin is a prescription antibiotic with the molecular formula C16H19N3O5S.

Which column and mobile phase are required for Amoxicillin analysis?

Run the separation on Primesep 100 (3.2 x 150 mm, 5 um, 100 A, dual ended), SKU 100-32.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.1%; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of Amoxicillin on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 0.5. Injection volume: 2. Run time: 17.

Sample Information

Structure of Amoxicillin
Analyte
Amoxicillin
Category
Antibiotic

Column Information

Primesep 100 column

Primesep 100

100-32.150.0510
Dimensions3.2 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025