Ascorbic Acid is a vitamin with the molecular formula C6H8O6. Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus fruits. Ascorbic Acid can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
The HPLC Method for Analysis of Ascorbic Acid on Alltesta™ Gradient is designed for Ascorbic Acid, a vitamin compound. Ascorbic Acid is a vitamin with the molecular formula C6H8O6.
Run the separation on Primesep SB (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU SB-46.150.0510. Typical mobile phase: MeCN, 50% 50% H3PO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 2. Run time: 5.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
