Acetylsalicylic acid (ASA), also known as Aspirin, is an organic compound with the molecular formula C9H8O4. It is a nonsteroidal anti-inflammatory drug (NSAID) that is used to reduce fever, pain, and inflammation. It is also used to treat Kawasaki disease, pericarditis, and rheumatic fever. Aspirin is a genetic medication that is widely available and can be procured without a prescription. It is one of the oldest NSAIDs in the world. Acetylsalicylic Acid (Aspirin) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Aspirin, Acetylsalicylic acid (ASA) on Alltesta™ Gradient is designed for Aspirin, Acetylsalicylic acid (ASA), a drug compound. Acetylsalicylic acid (ASA), also known as Aspirin, is an organic compound with the molecular formula C9H8O4.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 4.
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Last updated: Dec 3, 2025
