Biopterin is a petrin derivative with the chemical formula C9H11N5O3. As a cofactor for aromatic amino acid hydroxylases, it is a part of the synthesis of neurotransmitters like dopamine, serotonin, and norepinephrine. A biopterin imbalance can lead to a variety of disorders, most of which lead to neurological problems. Biopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Biopterin on Alltesta™ Gradient is designed for Biopterin, a organic compound compound. Biopterin is a petrin derivative with the chemical formula C9H11N5O3.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 5% 5% AmFm pH 3.0- 20 mM; H2O 95%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
