Apixaban is an anticoagulant medication with the chemical formula C25H25N5O4. It is used primarily to treat and prevent blood clots and stroke. It works through directly inhibiting factor Xa. In patients who have undergone knee or hop replacement surgery, apixaban helps prevent deep vein thrombosis. Apixaban can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Apixaban on Alltesta™ Gradient is designed for Apixaban, a drug compound. Apixaban is an anticoagulant medication with the chemical formula C25H25N5O4.
Run the separation on Primesep B (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU B-46.150.0510. Typical mobile phase: MeCN, 30% 30% AmFm pH 3.0- 20 mM; H2O 70%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 20. Run time: 9.5.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
