HPLC MethodAlltesta™ GradientPhenols275 nm

HPLC Method for Analysis of Guaiacol on Alltesta™ Gradient

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Chromatogram

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Chromatogram for Guaiacol
Flow Rate
1ml/min
Injection Volume
2µL
Run Time
5.5min
Detection
275 nm
Mobile Phase
H
H2O
60%
M
MeCN
40%
H
H2SO4
0.2%
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Frequently Asked Questions: HPLC Method for Analysis of Guaiacol on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Guaiacol on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Guaiacol on Alltesta™ Gradient is designed for Guaiacol, a phenols compound.

Which column and mobile phase are required for Guaiacol analysis?

Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 40% 40% H2SO4, 0.2%; H2O 60%.

What instrument conditions should I use for the HPLC Method for Analysis of Guaiacol on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 2. Run time: 5.5.

Sample Information

Structure of Guaiacol
Analyte
Guaiacol
Category
Phenols

Column Information

Primesep 100 column

Primesep 100

100-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Jan 7, 2026