Ibuprofen is a nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C13H18O2. It is used primarily to relieve pain, fever, and inflammation. There is numerous adverse effects to taking ibuprofen, including but not limited to nausea, heightened risk of hypertension, kidney impairment, and bronchospasm. Ibuprofen can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Ibuprofen on Alltesta™ Gradient is designed for Ibuprofen, a drug compound. Ibuprofen is a nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C13H18O2.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 10. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
