HPLC MethodAlltesta™ GradientDicarboximide fungicides275 nm

HPLC Method for Analysis of Iprodione on Alltesta™ Gradient

Iprodione is a hydantoin fungicide and nematicide with the chemical formula C13H13Cl2N3O3. It is used on a wide variety of crops that are affected by fungal diseases such as botrytis bunch rot, brown rot, and Sclerotinia. Approval of using it is different across the world. Iprodione can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

Chromatogram

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Chromatogram for Iprodione
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Flow Rate
1ml/min
Injection Volume
2µL
Run Time
4min
Detection
275 nm
Mobile Phase
M
MeCN
70%
H
H2O
30%
H
H2SO4
0.2%

Frequently Asked Questions: HPLC Method for Analysis of Iprodione on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Iprodione on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Iprodione on Alltesta™ Gradient is designed for Iprodione, a dicarboximide fungicides compound. Iprodione is a hydantoin fungicide and nematicide with the chemical formula C13H13Cl2N3O3.

Which column and mobile phase are required for Iprodione analysis?

Run the separation on Newcrom B (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU NB-46.150.0510. Typical mobile phase: MeCN, 70% 70% H2SO4, 0.2%; H2O 30%.

What instrument conditions should I use for the HPLC Method for Analysis of Iprodione on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 2. Run time: 4.

Sample Information

Structure of Iprodione
Analyte
Iprodione
Category
Dicarboximide fungicides

Column Information

Newcrom B column

Newcrom B

NB-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025