Isonicotinic Acid is an nucleoside with the C6H5NO2 molecular formula. It is a derivative of pyridine, making it a pyridinemonocarboxylic acid. It acts primarily as a metabolite in humans and algae. Isonicotinic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Isonicotinic Acid on Alltesta™ Gradient is designed for Isonicotinic Acid, a acid compound. Isonicotinic Acid is an nucleoside with the C6H5NO2 molecular formula.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 30% 30% H2SO4, 0.1%; H2O 70%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 3. Run time: 7.
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Last updated: Dec 3, 2025
