Metoprolol is a medication with the chemical formula C15H25NO3. It is primarily used to treat angina, high blood pressure, abnormally fast heart rate, and to prevent heart problems after a myocardial infraction. It works through blocking β1-adrenergic receptors, which increase heartrate. Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Metoprolol on Alltesta™ Gradient is designed for Metoprolol, a drug compound. Metoprolol is a medication with the chemical formula C15H25NO3.
Run the separation on Primesep 200 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 200-46.150.0510. Typical mobile phase: MeCN, 50% 50% H3ЗO4, 0.5%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 5.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
