Metronidazole is an antibiotic with C6H9N3O3 molecular formula. It works strongly against anaerobic bacteria and protozoa. It is available as oral tablets, capsules, topical gels, and injections. To truly kill bacteria and prevent infection, a full course of the medication must be completed as prescribed by a doctor. Metronidazole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Metronidazole on Alltesta™ Gradient is designed for Metronidazole, a antibiotic compound. Metronidazole is an antibiotic with C6H9N3O3 molecular formula.
Run the separation on Newcrom R1 (4.6x150 mm, 5 um, 100A, dual ended), SKU NR1-46.150.0510. Typical mobile phase: MeCN, 5% 5% H3PO4, 0.1%; H2O 95%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 8.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
