Neopterin is an pteridine with the chemical formula C9H11N5O4. It is indicative of a pro-inflammatory immune status, therefore it typically serves as a marker of the activation of the cellular immune system. Neopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Neopterin on Alltesta™ Gradient is designed for Neopterin, a organic compound compound. Neopterin is an pteridine with the chemical formula C9H11N5O4.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 5% 5% AmFm pH 3.0- 20 mM; H2O 95%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
