Nicotinamide, also known as niacinaminde, is a form of vitamin B3 with the chemical formula C6H6N2O2. It is preferred treatment for pellagra and is a common treatment for acne due to it’s anti-inflammatory properties. If consumed daily, it is shown to decrease the risk of skin cancers other than melanoma. Nicotinamide can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Nicotinamide on Alltesta™ Gradient is designed for Nicotinamide, a vitamin compound. Nicotinamide, also known as niacinaminde, is a form of vitamin B3 with the chemical formula C6H6N2O2.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
