Oligonucleotide 5A is a small RNA molecule with a specific adenine sequence. They are primarily used to inhibit viral and cancer cell growth. In RNA synthesis, they are intermediates in enzymatic synthesis of capped RNAs. Oligonucleotide 5A can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Oligonucleotide 5A on Alltesta™ Gradient is designed for Oligonucleotide 5A, a nucleotide base compound. Oligonucleotide 5A is a small RNA molecule with a specific adenine sequence.
Run the separation on Chromni™ (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU CHR-46.150.0510.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 5. Run time: 8.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
