HPLC MethodAlltesta™ GradientAcid275 nm

HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient

Phthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂. It consists of a benzene ring with two carboxylic acid groups (-COOH) attached to adjacent carbon atoms (in the ortho position). It is mainly produced by the oxidation of naphthalene or o-xylene. In the form of phthalic anhydride, it is used as an industrial chemical. It is primarily used to make phthalates, which are esters of phthalic acid. Phthalic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

Chromatogram

Zoom Available
Chromatogram for Phthalic acid
Loading interactive viewer...
Flow Rate
1ml/min
Injection Volume
1µL
Run Time
3min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
H
H2SO4
0.2%

Frequently Asked Questions: HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient is designed for Phthalic acid, a acid compound. Phthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂.

Which column and mobile phase are required for Phthalic acid analysis?

Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 3.

Sample Information

Structure of Phthalic acid
Analyte
Phthalic acid
Category
Acid

Column Information

Primesep 100 column

Primesep 100

100-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
View Product

Need Method Support?

Our application team can help you adapt this method or develop a new one for your specific needs.

Last updated: Dec 3, 2025