HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient
Phthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂. It consists of a benzene ring with two carboxylic acid groups (-COOH) attached to adjacent carbon atoms (in the ortho position). It is mainly produced by the oxidation of naphthalene or o-xylene. In the form of phthalic anhydride, it is used as an industrial chemical. It is primarily used to make phthalates, which are esters of phthalic acid. Phthalic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Frequently Asked Questions: HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient
What analyte does the HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient cover?
The HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient is designed for Phthalic acid, a acid compound. Phthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂.
Which column and mobile phase are required for Phthalic acid analysis?
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
What instrument conditions should I use for the HPLC Method for Analysis of Phthalic acid on Alltesta™ Gradient?
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 3.