Prednisolone is a steroid hormone with the molecular formula C21H28O5. It is a synthetic version of natural hormones as well as a corticosteroid medication and is used to reduce inflammation and suppress the immune system. Some of the conditions that it treats include: allergic reactions, autoimmune diseases. respiratory illnesses, skin conditions, and certain blood disorders. Prednisolone can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
The HPLC Method for Analysis of Prednisolone on Alltesta™ Gradient is designed for Prednisolone, a synthetic hormone compound. Prednisolone is a steroid hormone with the molecular formula C21H28O5.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 5. Run time: 5.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
