HPLC MethodAlltesta™ GradientHydrophobic, Herbicide, Pesticide275 nm

HPLC Method for Analysis of Prochloraz on Alltesta™ Gradient

Prochloraz is an imidazole fungicide with the chemical formula C15H16Cl3N3O2. It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase. Prochloraz can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

Chromatogram

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Chromatogram for Prochloraz
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Flow Rate
1ml/min
Injection Volume
1µL
Run Time
8min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
A
AmFm pH 3.0 - 20 mM

Frequently Asked Questions: HPLC Method for Analysis of Prochloraz on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Prochloraz on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Prochloraz on Alltesta™ Gradient is designed for Prochloraz, a hydrophobic, herbicide, pesticide compound. Prochloraz is an imidazole fungicide with the chemical formula C15H16Cl3N3O2.

Which column and mobile phase are required for Prochloraz analysis?

Run the separation on Newcrom B (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU NB-46.150.0510. Typical mobile phase: MeCN, 50% 50% AmFm pH 3.0 - 20 mM; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of Prochloraz on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 8.

Sample Information

Structure of Prochloraz
Analyte
Prochloraz
Category
Hydrophobic, Herbicide, Pesticide

Column Information

Newcrom B column

Newcrom B

NB-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025