Propranolol is an organic compound with the molecular formula C16H21NO2. It is primarily used to treat hypertension, angina, migraine headaches, and hypertrophic subaortic stenosis. As a beta-blocker, it works through affecting nerve impulse responses. You can find detailed UV spectra of Propranolol and information about its various lambda maxima by visiting the following link. Propranolol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Propranolol on Alltesta™ Gradient is designed for Propranolol, a phenols compound. Propranolol is an organic compound with the molecular formula C16H21NO2.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 70% 70% H2SO4, 0.2%; H2O 30%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 3.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
