Simazine is an herbicide with the molecular formula C7H12ClN5. Typically, it is used to control various annual weeds in established crops. It is a pre-emergence herbicide, meaning that it has to be applied prior to the weeds emerging, as it is ineffective against grown plants Simazine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
The HPLC Method for Analysis of Simazine on Alltesta™ Gradient is designed for Simazine, a herbicide compound. Simazine is an herbicide with the molecular formula C7H12ClN5.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 5. Run time: 8.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
