trans-Zeatin is a cytokinin derived from adenine with two forms, cis- and trans-isomer. It has the chemical formula C10H13N5O. It promotes callus initiation and fruit set, delays yellowing for vegetables, and causes auxiliary stems to grow. You can find detailed UV spectra of trans-Zeatin and information about its various lambda maxima by visiting the following link. trans-Zeatin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with sulfuric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of trans-Zeatin on Alltesta™ Gradient is designed for trans-Zeatin, a cytokinin compound. trans-Zeatin is a cytokinin derived from adenine with two forms, cis- and trans-isomer.
Run the separation on Primesep 100 (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU 100-46.150.0510. Typical mobile phase: MeCN, 40% 40% H2SO4, 0.1%; H2O 60%.
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 1. Injection volume: 1. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Jan 7, 2026
