HPLC Method for Analysis of Tyramine on Alltesta™ Gradient
Tyramine is an amine compound with the molecular formula C8H11NO. As a monoamine oxidase (MAO) substrate, it is broken down by the enzyme MAO in the body. It is typically found in fermented, aged, smoked, and dried foods. Tyramine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Frequently Asked Questions: HPLC Method for Analysis of Tyramine on Alltesta™ Gradient
What analyte does the HPLC Method for Analysis of Tyramine on Alltesta™ Gradient cover?
The HPLC Method for Analysis of Tyramine on Alltesta™ Gradient is designed for Tyramine, a amine compound. Tyramine is an amine compound with the molecular formula C8H11NO.
Which column and mobile phase are required for Tyramine analysis?
Run the separation on Primesep 100 (3.2 x 150 mm, 5 um, 100 A, dual ended), SKU 100-32.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
What instrument conditions should I use for the HPLC Method for Analysis of Tyramine on Alltesta™ Gradient?
Operate on Alltesta™ Gradient with 275 nm. Flow rate: 0.5. Injection volume: 2. Run time: 10.