HPLC MethodAlltesta™ GradientAmine275 nm

HPLC Method for Analysis of Tyramine on Alltesta™ Gradient

Tyramine is an amine compound with the molecular formula C8H11NO. As a monoamine oxidase (MAO) substrate, it is broken down by the enzyme MAO in the body. It is typically found in fermented, aged, smoked, and dried foods. Tyramine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Chromatogram

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Chromatogram for Tyramine
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Flow Rate
0.5ml/min
Injection Volume
2µL
Run Time
10min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
H
H2SO4
0.2%

Frequently Asked Questions: HPLC Method for Analysis of Tyramine on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Tyramine on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Tyramine on Alltesta™ Gradient is designed for Tyramine, a amine compound. Tyramine is an amine compound with the molecular formula C8H11NO.

Which column and mobile phase are required for Tyramine analysis?

Run the separation on Primesep 100 (3.2 x 150 mm, 5 um, 100 A, dual ended), SKU 100-32.150.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of Tyramine on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with 275 nm. Flow rate: 0.5. Injection volume: 2. Run time: 10.

Sample Information

Structure of Tyramine
Analyte
Tyramine
Category
Amine

Column Information

Primesep 100 column

Primesep 100

100-32.150.0510
Dimensions3.2 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025