6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation. 6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
The HPLC Method for Analysis of 6-Benzylaminopurine on Cromite™ is designed for 6-Benzylaminopurine, a synthetic cytokinin compound. 6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5.
Run the separation on Newcrom R1 (3.2 x 115 mm, 5 um, 100 A, dual ended), SKU NR1-32.115.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.2%; H2O 50%.
Operate on Cromite™ with 275 nm. Flow rate: 0.5. Injection volume: 1. Run time: 10.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
