HPLC MethodCromite™Nucleotide Base275 nm

HPLC Method for Analysis of Adenine on Cromite™

Adenine is a chemical compound with the molecular formula C5H5N5. It is a building block for DNA and RNA as one of the four essential nucleotide bases. It is vital as a part of the main energy-carrying molecule in cells. When in DNA, it pairs with thymine, while in RNA, it pairs with uracil. Adenine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

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Flow Rate

0.7ml/min

Injection Volume

2µL

Run Time

5min

Detection

275 nm

Mobile Phase

MeCN

50%

H₂O

50%

H₂SO₄

0.1%

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Frequently Asked Questions: HPLC Method for Analysis of Adenine on Cromite™

What analyte does the HPLC Method for Analysis of Adenine on Cromite™ cover?

The HPLC Method for Analysis of Adenine on Cromite™ is designed for Adenine, a nucleotide base compound. Adenine is a chemical compound with the molecular formula C5H5N5.

Which column and mobile phase are required for Adenine analysis?

Run the separation on Primesep 100 (3.2 x 115 mm, 5 um, 100 A, dual ended), SKU 100-32.115.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.1%; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of Adenine on Cromite™?

Operate on Cromite™ with 275 nm. Flow rate: 0.7. Injection volume: 2. Run time: 5.

Sample Information

Analyte

Adenine

Column Information

Primesep 100

100-32.115.0510

Dimensions3.2 x 115 mm

Particle5 µm

Pore Size100 Å

Typedual ended

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Last updated: Dec 3, 2025