Butylated Hydroxyanisole (BHA) is a petrochemical with the chemical formula C11H16O2. Due to it’s antioxidant properties, it is used in preservation of food, cosmetics, and pharmaceuticals. Due to previous experiments in animals, it is suspected to be a carcinogen for humans. Butylated Hydroxyanisole (BHA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Butylated Hydroxyanisole (BHA) on Cromite™ is designed for Butylated Hydroxyanisole (BHA), a phenolic antioxidants compound. Butylated Hydroxyanisole (BHA) is a petrochemical with the chemical formula C11H16O2.
Run the separation on Newcrom R1 (3.2 x 100 mm, 5 um, 100 A, dual ended), SKU NR1-32.115.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.1%; H2O 50%.
Operate on Cromite™ with 275 nm. Flow rate: 0.5. Injection volume: 5. Run time: 7.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Dec 3, 2025
