What analyte does the HPLC Method for Analysis of Simazine on Cromite™ cover?
The HPLC Method for Analysis of Simazine on Cromite™ is designed for Simazine, a herbicide compound. Simazine is an herbicide with the molecular formula C7H12ClN5.
HPLC MethodCromite™Herbicide275 nm
HPLC Method for Analysis of Simazine on Cromite™
Simazine is an herbicide with the molecular formula C7H12ClN5. Typically, it is used to control various annual weeds in established crops. It is a pre-emergence herbicide, meaning that it has to be applied prior to the weeds emerging, as it is ineffective against grown plants Simazine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Chromatogram
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Flow Rate
0.7ml/min
Injection Volume
2µL
Run Time
5min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
H
H2SO4
0.1%
Frequently Asked Questions: HPLC Method for Analysis of Simazine on Cromite™
Which column and mobile phase are required for Simazine analysis?
Run the separation on Primesep 100 (4.6 x 50 mm, 5 um, 100 A, dual ended), SKU 100-32.115.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.1%; H2O 50%.
What instrument conditions should I use for the HPLC Method for Analysis of Simazine on Cromite™?
Operate on Cromite™ with 275 nm. Flow rate: 0.7. Injection volume: 2. Run time: 5.
Sample Information
Analyte
Simazine
Category
Herbicide
Column Information
Primesep 100
100-32.115.0510
Dimensions4.6 x 50 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025