What analyte does the HPLC Method for Analysis of Tyramine on Cromite™ cover?
The HPLC Method for Analysis of Tyramine on Cromite™ is designed for Tyramine, a amine compound. Tyramine is an amine compound with the molecular formula C8H11NO.
HPLC MethodCromite™Amine275 nm
HPLC Method for Analysis of Tyramine on Cromite™
Tyramine is an amine compound with the molecular formula C8H11NO. As a monoamine oxidase (MAO) substrate, it is broken down by the enzyme MAO in the body. It is typically found in fermented, aged, smoked, and dried foods. Tyramine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Chromatogram
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Flow Rate
0.7ml/min
Injection Volume
2µL
Run Time
5min
Detection
275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
H
H2SO4
0.1%
Frequently Asked Questions: HPLC Method for Analysis of Tyramine on Cromite™
Which column and mobile phase are required for Tyramine analysis?
Run the separation on Primesep 100 (3.2 x 115 mm, 5 um, 100 A, dual ended), SKU 100-32.115.0510. Typical mobile phase: MeCN, 50% 50% H2SO4, 0.1%; H2O 50%.
What instrument conditions should I use for the HPLC Method for Analysis of Tyramine on Cromite™?
Operate on Cromite™ with 275 nm. Flow rate: 0.7. Injection volume: 2. Run time: 5.
Sample Information
Analyte
Tyramine
Category
Amine
Column Information
Primesep 100
100-32.115.0510
Dimensions3.2 x 115 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Dec 3, 2025