HPLC MethodAlltesta™ GradientAcidUV, 275 nm

HPLC Method for Analysis of Benzoic Acid on Alltesta™ Gradient

Chromatogram

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Chromatogram for Benzoic Acid
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Flow Rate
0.5ml/min
Injection Volume
2µL
Run Time
6min
Detection
UV, 275 nm
Mobile Phase
M
MeCN
80%
H
H2O
20%
H
H2SO4
0.2%

Frequently Asked Questions: HPLC Method for Analysis of Benzoic Acid on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Benzoic Acid on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Benzoic Acid on Alltesta™ Gradient is designed for Benzoic Acid, a acid compound.

Which column and mobile phase are required for Benzoic Acid analysis?

Run the separation on Newcrom BH (3.2 x 100 mm, 5 um, 100 A, dual ended), SKU NBH-32.100.0510. Typical mobile phase: MeCN, 80% 80% H2SO4, 0.2%; H2O 20%.

What instrument conditions should I use for the HPLC Method for Analysis of Benzoic Acid on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with UV, 275 nm. Flow rate: 0.5. Injection volume: 2. Run time: 6.

Sample Information

Structure of Benzoic Acid
Analyte
Benzoic Acid
Category
Acid

Column Information

Newcrom BH column

Newcrom BH

NBH-32.100.0510
Dimensions3.2 x 100 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Apr 23, 2026